ASTM E3143-18b
- Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes
- standard by ASTM International, 11/15/2018
- Category: ASTM
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1.1 This practice covers procedures for vitrifying and recording images of a suspension of liposomes with a cryo-transmission electron microscope (cryo-TEM) for the purpose of evaluating their shape, size distribution and lamellarity for quality assessment. The sample is vitrified in liquid ethane onto specially prepared holey, ultra-thin, or continuous carbon TEM grids, and imaged in a cryo-holder placed in a cryo-TEM.
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